Composite

Part:BBa_K676008

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


GyrA cassette

This expression cassette is regulated by an IPTG inducible promoter and will be expressing Gyr A subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. However in order to introduce negative supercoiling into the DNA , a functional tetrameric gyrase structure with two GyrA and two GyrB should form with the presence of divalent magnesium ions and ATP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1819
    Illegal BglII site found at 2764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
    Illegal AgeI site found at 929
    Illegal AgeI site found at 986
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 2381
    Illegal AgeI site found at 2749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 269
    Illegal SapI.rc site found at 277
    Illegal SapI.rc site found at 1736


[edit]
Categories
Parameters
None